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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse TNFR2/CD120b protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0683

Validation Data Gallery

  • Purity of Recombinant Mouse TNFR2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse TNFR2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse TNFR2 protein Val23-Gly258 (Accession# Q545P4) with a His tag at the C-terminus.
GeneID 21938
Accession Q545P4
PredictedSize 26.4 kDa
SDS-PAGE 40-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TNFR2, also known as CD120b, TNFRSF1B or p75, is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. Tumor necrosis factor (TNF, TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through TNFRSF1A and TNFRSF1B. TNFRSF1A is widely expressed, whereas TNFRSF1B exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells. Both TNFRSF1A and TNFRSF1B can also exist in soluble forms, probably derived by proteolytic cleavage from the cell surface forms. Various defects in the TNFRSF1B pathway, due to polymorphisms in the TNFRSF1B gene, upregulated expression of TNFRSF1B and TNFRSF1B shedding, have been implicated in the pathology of several autoimmune disorders.

References:

1. Islam A, et al. (2006) J Biol Chem. 281 (10):6860-6873.
2. Faustman D, et al. (2010) Nat Rev Drug Discov. 9 (6):482-493.
3. Cabal-Hierro L, et al. (2012) Cell Signal. 24 (6):1297-1305.
4. Diez-Ruiz A, et al. (1995) Eur J Haematol. 54 (1):1-8.