Speedy™ Mouse Timp2 One-Step ELISA Kit
Sensitivity
11.8 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : SE60073
Synonyms
Metalloproteinase inhibitor 2, Timp-2, Tissue inhibitor of metalloproteinases 2
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Timp2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 11.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21858 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 88% | 82%-98% |
| Cell culture supernatant | 92% | 85%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,475.8 | 31.3 | 1.3 |
| 2 | 8 | 620.6 | 9.4 | 1.5 |
| 3 | 8 | 307.1 | 2.9 | 0.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,475.8 | 58.9 | 2.4 |
| 2 | 16 | 614.8 | 13.1 | 2.1 |
| 3 | 16 | 309.3 | 5.9 | 1.9 |
Background Information
The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Metalloproteinase inhibitor 2, Timp-2, Tissue inhibitor of metalloproteinases 2 |