Speedy™ Mouse Tie-2 One-Step ELISA Kit
Sensitivity
2.3 pg/mL
Range
93.8-6000 pg/mL
Reactivity
Mouse
Cat no : SE60026
Synonyms
Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Tie-2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21687 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 108% | 105%-114% |
| Cell culture supernatant | 93% | 90%-97% |
| Tissue homogenates | 98% | 94%-103% |
| Tissue lysate | 89% | 82%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,086.5 | 71.5 | 2.3 |
| 2 | 8 | 702.0 | 11.3 | 1.6 |
| 3 | 8 | 367.8 | 6.3 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,066.4 | 72.1 | 2.4 |
| 2 | 16 | 703.0 | 23.9 | 3.4 |
| 3 | 16 | 358.6 | 14.3 | 4.0 |
Background Information
Tie-2 (also known as TEK or CD202b) is a receptor tyrosine kinase (RTK) critical for vascular development, angiogenesis, and endothelial cell function. Tie-2 is essential for blood vessel maturation. Genetic knockout of Tie-2 or Ang-1 in mice leads to embryonic lethality due to defects in cardiac development and vascular remodeling. Ligand-induced Tie-2 phosphorylation activates PI3K-AKT (promoting survival) and MAPK pathways (regulating proliferation and migration).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK |