Speedy™ Mouse TNF-alpha One-Step ELISA Kit
Sensitivity
0.2 pg/mL
Range
1.95-125 pg/mL
Reactivity
Mouse
Cat no : SE60082
Synonyms
Tnf, Tnfa, DIF, TNF a, TNF alpha
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse TNF-alpha One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 1.95-125 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21926 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 90%-116% |
| Cell culture supernatant | 116% | 106%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 70.6 | 2.1 | 3.0 |
| 2 | 8 | 15.1 | 0.5 | 3.3 |
| 3 | 8 | 7.0 | 0.4 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 68.0 | 3.3 | 4.9 |
| 2 | 16 | 14.8 | 0.6 | 4.1 |
| 3 | 16 | 7.0 | 0.6 | 8.6 |
Background Information
TNF, also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Tnf, Tnfa, DIF, TNF a, TNF alpha |