Recombinant Mouse TIM-3 protein (rFc Tag)(HPLC verified)
Species
Mouse
Purity
>90 %, SDS-PAGE
>90 %, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg2831
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse TIM-3 protein Ser21-Thr189 (Accession# Q8VIM0-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 171285 |
| Accession | Q8VIM0-1 |
| PredictedSize | 44.3 kDa |
| SDS-PAGE | 52-70 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
TIM3, also known as HAVCR2, is a member of T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection. TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity.
References:
1. Isabel Sada-Ovalle, et al. (2012) J Immunol. 189(12):5896-902. 2. Chen Zhu, et al. (2005) Nat Immunol. 6(12):1245-52.