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Recombinant Mouse SLPI protein (mFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

mFc Tag

Activity

not tested

Cat no : Eg4190

Validation Data Gallery

  • Purity of Recombinant Mouse SLPI was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse SLPI was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse SLPI was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse SLPI was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse SLPI protein Gly26-Met131 (Accession# P97430) with a mouse IgG Fc tag at the N-terminus.
GeneID 20568
Accession P97430
PredictedSize 39.4 kDa
SDS-PAGE 38-43 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SLPI, a member of Whey acidic protein family, could mediate a variety of biological processes including cell proliferation and apoptosis, repairing reaction and immune response, with crucial roles in anti-protease, anti-inflammatory, anti-bacterial and anti-virus. SLPI is mainly expressed in epithelial cells.

References:

1. Vafadarnejad E. et al. (2020) Circ Res. 127(9):e232-e249. 
2. Xu W. et al. (2023) Neuron. 111(20):3244-3254.e8.
3. Brown R. et al. (2024) Front Immunol. 15:1433642. 
4. Morimoto A. et al. (2021) mun. 12(1):2136.
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