Recombinant Mouse Antithrombin III protein (mFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
mFc Tag
Activity
not tested
Cat no : Eg3967
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Antithrombin III protein His33-Asn465 (Accession# P32261) with a mouse IgG Fc tag at the N-terminus. |
| GeneID | 11905 |
| Accession | P32261 |
| PredictedSize | 76.6 kDa |
| SDS-PAGE | 75-85 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Serpin C1 is a plasma protease inhibitor belonging to the serpin superfamily. It plays a critical role in regulating the blood coagulation cascade and the complement system by inhibiting various activated serine proteases of the coagulation system, including thrombin. It is synthesized in the liver and secreted into the bloodstream, circulating as an inhibitor of serine proteases. Serpin C1 is the major control serpin of the kallikrein-kinin system (KKS), and its deficiency can lead to uncontrolled activation of KKS, resulting in the generation of the vasoactive peptide bradykinin, which is associated with hereditary angioedema (HAE).
References:
1.Drouet C. et al. (2022). Front Allergy. 3:835503. 2.Ponard D. et al. (2020). Hum Mutat. 41(1):38-57.