Recombinant Mouse SHH protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0657
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse SHH protein Cys25-Gly198 (Accession# Q62226, Arg29His) with a His tag at the C-terminus. |
| GeneID | 20423 |
| Accession | Q62226 |
| PredictedSize | 20.6 kDa |
| SDS-PAGE | 19-21 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Sonic Hedgehog (Shh) is a fundamental secreted glycoprotein that acts as a key morphogen during embryonic development and plays complex roles in adult tissue homeostasis and disease. Shh is a master regulator of cell fate, proliferation, and survival. Dysregulation of the Shh pathway is a direct cause of several severe conditions, making it a prime therapeutic target.
References:
1. Samkari, Ayman et al. Expert review of neurotherapeutics vol. 15,7 (2015): 763-70. 2. Lopez-Rios, Javier. Seminars in cell & developmental biology vol. 49 (2016): 116-24. 3. Wang, Xiang-Zhi et al. Journal of the Chinese Medical Association : JCMA vol. 82,5 (2019): 343-350.