Speedy™ Mouse Resistin One-Step ELISA Kit
Sensitivity
1.7 pg/mL
Range
39-2500 pg/mL
Reactivity
Mouse
Cat no : SE60025
Synonyms
Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Resistin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57264 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse plasma | 112% | 106%-121% |
| Cell culture supernatant | 107% | 101%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,145.5 | 36.2 | 3.2 |
| 2 | 8 | 282.6 | 6.6 | 2.3 |
| 3 | 8 | 150.6 | 3.4 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,141.7 | 47.2 | 4.1 |
| 2 | 16 | 282.4 | 5.5 | 2.0 |
| 3 | 16 | 153.2 | 4.5 | 2.9 |
Background Information
Resistin (or 'resistance to insulin') was originally discovered in mice in 2001 and named for its ability to resist (interfere with) insulin action; at that time, it was proposed as a link between obesity and diabetes. Mouse resistin is an 11 kDa cysteine-rich polypeptide, and its gene is located on chromosome 8. It is synthesized as a 114 amino acid precursor, with a 20 aa signal sequence and a 94 aa mature segment. Neuroprotective nature of adipokine resistin in the early stages of focal cerebral ischemia in a stroke mouse model suggests that resistin may have a therapeutic potential effect in management of neurodegenerative disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3 |