Mouse Resistin ELISA Kit
Sensitivity
4.5 pg/mL
Range
39-2500 pg/mL
Reactivity
Mouse
Cat no : KE10205
Synonyms
Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3
Validation Data Gallery
Product Information
KE10205 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Resistin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Resistin. The assay recognizes mouse Resistin. An antibody specific for mouse Resistin has been pre-coated onto the microwells. The mouse Resistin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Resistin is added to detect the captured mouse Resistin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Resistin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 4.5 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57264 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 87% | 82%-93% |
| Cell culture supernatant | 106% | 102%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,199.6 | 59.0 | 4.9 |
| 2 | 8 | 482.5 | 23.4 | 4.8 |
| 3 | 8 | 307.8 | 15.6 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,153.7 | 81.0 | 7.0 |
| 2 | 16 | 469.9 | 25.1 | 5.3 |
| 3 | 16 | 298.7 | 19.1 | 6.4 |
Background Information
Resistin (or 'resistance to insulin') was originally discovered in mice in 2001 and named for its ability to resist (interfere with) insulin action; at that time, it was proposed as a link between obesity and diabetes. Mouse resistin is an 11 kDa cysteine-rich polypeptide, and its gene is located on chromosome 8. It is synthesized as a 114 amino acid precursor, with a 20 aa signal sequence and a 94 aa mature segment. Neuroprotective nature of adipokine resistin in the early stages of focal cerebral ischemia in a stroke mouse model suggests that resistin may have a therapeutic potential effect in management of neurodegenerative disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3 |