Speedy™ Mouse RAGE One-Step ELISA Kit
Sensitivity
1.4 pg/mL
Range
31.25-2000 pg/mL, 62.5-4000 pg/mL
Reactivity
Mouse
Cat no : SE60031
Synonyms
Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse RAGE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL, 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11596 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 112% | 106%-116% |
| Cell culture supernatant | 106% | 98%-117% |
| Tissue lysate | 88% | 78%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,026.4 | 12.8 | 1.2 |
| 2 | 8 | 252.9 | 1.5 | 0.6 |
| 3 | 8 | 130.1 | 2.0 | 1.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,034.6 | 16.0 | 1.5 |
| 2 | 16 | 254.2 | 4.2 | 1.7 |
| 3 | 16 | 132.3 | 3.0 | 2.3 |
Background Information
RAGE (receptor for advanced glycation endproducts), also called AGER, is a transmembrane receptor of the immunoglobulin super family. It mediates interactions of advanced glycosylation end products (AGE) which accumulate in vascular tissue in aging in diabetes. Acts as a mediator of vascular inflammation such as atherosclerosis and a complication of diabetes. AGE/RAGE signal regulates production or expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation. Interaction with S100B after myocardial infarction regulates myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. ABPP-initiated RAGE signaling, especially stimulation of P38 mitogen-activated protein kinase (MAPK), delivers ABPP as a complex with RAGE to the intraneuronal. RAGE has higher expression in lung tissues, in particular in alveolar type I cells, and is lost in idiopathic pulmonary fibrosis (IPF).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1 |