Recombinant Mouse Pon2 protein (rFc Tag)
Species
Mouse
Purity
>80 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2741
Validation Data Gallery
Product Information
| Purity | >80 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse PON2 protein Ala30-Leu354 (Accession# Q62086) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 330260 |
| Accession | Q62086 |
| PredictedSize | 62.1 kDa |
| SDS-PAGE | 58-75 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain.
References:
1. Giordano, Gennaro et al. Toxicology and applied pharmacology vol. 256,3 (2011): 369-78. 2. Costa, Lucio G et al. Neurotoxicology vol. 43 (2014): 3-9. 3. Aviram, Michael, and Mira Rosenblat. Free radical biology & medicine vol. 37,9 (2004): 1304-16.