Speedy™ Mouse PD-1/CD279 One-Step ELISA Kit
Sensitivity
2.3 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Mouse
Cat no : SE60029
Synonyms
CD279, PD1, PD-1, PD 1, Pdc1
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse PD-1/CD279 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18566 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 102% | 93%-111% |
| Cell lysate | 102% | 94%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,361.2 | 62.5 | 2.6 |
| 2 | 8 | 553.6 | 9.2 | 1.7 |
| 3 | 8 | 277.1 | 3.7 | 1.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,334.2 | 55.7 | 2.4 |
| 2 | 16 | 554.6 | 10.3 | 1.9 |
| 3 | 16 | 277.1 | 6.7 | 2.4 |
Background Information
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acids type I transmembrane protein composed of a Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM), as well as an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 can be expressed on activated T cells, B cells, natural killer T cells, monocytes, and dendritic cells (DCs). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD279, PD1, PD-1, PD 1, Pdc1 |