Speedy™ Mouse P-Selectin/CD62P One-Step ELISA Kit
Sensitivity
8.5 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Mouse
Cat no : SE60019
Synonyms
CD62P, P-selectin, Grmp, P selectin, PADGEM
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse P-Selectin/CD62P One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 8.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20344 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 100% | 95%-110% |
| Cell culture supernatant | 96% | 89%-101% |
| Tissue lysate | 98% | 91%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,051.8 | 19.1 | 1.8 |
| 2 | 8 | 252.8 | 8.0 | 3.2 |
| 3 | 8 | 65.5 | 1.6 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,046.5 | 19.6 | 1.9 |
| 2 | 16 | 251.4 | 6.9 | 2.7 |
| 3 | 16 | 65.4 | 1.8 | 2.8 |
Background Information
P-sectieln, also named as SELP, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62P, P-selectin, Grmp, P selectin, PADGEM |