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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse MGL1/CD301a protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2698

Validation Data Gallery

  • Purity of Recombinant Mouse MGL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse MGL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse MGL1/CD301a was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse MGL1/CD301a was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse MGL1 protein Gln57-Ser304 (Accession# P49300) with a rabbit IgG Fc tag at the C-terminus.
GeneID 17312
Accession P49300
PredictedSize 54.3 kDa
SDS-PAGE 50-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Macrophage galactose-type lectin (MGL) is a type II transmembrane glycoprotein belonging to the type II family of C-type lectin receptors (CLR). MGL is expressed on professional antigen presenting cells. In mice,there are two homologs of human MGL, MGL1(CD301a), and MGL2(CD301b). They contain the Gln–Pro–Asp (QPD) sequence in their carbohydrate recognition domain that facilitates galactose or N-acetylgalactosamine (GalNAc) recognition. Murine MGL1 was found to be highly specific for Lewis X and Lewis A structures, whereas mMGL2 recognized N-actetylgalactosamine (GalNAc) and galactose.

References:

1.Singh SK, et al. (2009) Mol Immunol. 46(6):1240-9. 
2.Denda-Nagai K, et al. (2010) J Biol Chem. 18;285(25):19193-204. 
3.Zizzari IG, et al. (2015) J Immunol Res. 2015:450695.