Speedy™ Mouse Mesothelin One-Step ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.156-10 ng/mL
Reactivity
Mouse
Cat no : SE60062
Synonyms
Megakaryocyte-potentiating factor, Mes, Mesothelin, cleaved form, Mpf, Msln
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Mesothelin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56047 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 84%-97% |
| Cell lysate | 95% | 89%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.11 | 0.05 | 0.98 |
| 2 | 8 | 1.30 | 0.03 | 2.31 |
| 3 | 8 | 0.70 | 0.02 | 2.86 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.12 | 0.08 | 1.56 |
| 2 | 16 | 1.32 | 0.04 | 3.03 |
| 3 | 16 | 0.69 | 0.02 | 2.90 |
Background Information
Mesothelin is a 40-kDa cell surface glycoprotein that is present on normal mesothelial cells lining the pleura, peritoneum and pericardium. Mesothelin expression in normal human tissues is observed only in a single layer of mesothelial cells lining the pleura, peritoneum and pericardium, surface epithelial cells of the ovary, tunica vaginalis, rete testis and the tonsilar and fallopian tube epithelial cells. However, mesothelin is highly expressed in several cancers, including epitheloid mesotheliomas, pancreatic, biliary adenocarcinomas, gastric and ovarian cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Megakaryocyte-potentiating factor, Mes, Mesothelin, cleaved form, Mpf, Msln |