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Recombinant Mouse LY75/DEC-205 protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2649

Validation Data Gallery

  • Purity of Recombinant Mouse LY75 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse LY75 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • The purity of Mouse LY75/DEC-205 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse LY75/DEC-205 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90%, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse LY75 protein Ser28-Asp1667 (Accession# Q60767) with a rabbit IgG Fc tag at the C-terminus.
GeneID 17076
Accession Q60767
PredictedSize 214.1 kDa
SDS-PAGE 200-300 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ly75 (Lymphocyte Antigen 75), also known as DEC205/CD205, is a type I transmembrane protein belonging to the C-type lectin receptor family. Ly75 is expressed in immune cells and cancers, and participates in tumor immunity through a key role in endocytosis by major histocompatibility complex (MHC) molecules and in antigen presentation to T-cells. Ly75 can regulate EOC cell phenotype and transfer potential. 75 regulates EOC cell phenotype and metastatic potential and has a direct role in regulating Wnt/β-cyclin pathway activity in epithelial ovarian cancer (EOC) cells. (PMID: 32156068)

References:

1. Mehdi S, et al. Int J Mol Sci. 2020;21(5):1848.                                                       
2. Mehdi S,et al. Int J Mol Sci. 2020;21(14):4992.                                                          
3. Gil M, et al. J Clin Med. 2020;9(5):1383.
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