Speedy™ Mouse Lipocalin-2/NGAL One-Step ELISA Kit
Sensitivity
0.6 pg/mL
Range
6.25-400 pg/mL
Reactivity
Mouse
Cat no : SE60004
Synonyms
Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Lipocalin-2/NGAL One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 6.25-400 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16819 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 99% | 94%-105% |
| Urine | 97% | 90%-103% |
| Mouse serum | 100% | 96%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 106.6 | 3.3 | 3.1 |
| 2 | 8 | 26.1 | 1.0 | 3.7 |
| 3 | 8 | 13.2 | 0.4 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 105.3 | 3.4 | 3.2 |
| 2 | 16 | 25.8 | 0.8 | 3.0 |
| 3 | 16 | 13.2 | 0.9 | 6.5 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL were identified, including iron trafficking, chemotactic, and promoting cell differentiation and proliferation. NGAL was originally found in neutrophils and expressed in tissues containing renal, uterus, liver, spleen, cardiomyocytes, neurons, and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, and metabolic diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3 |