Recombinant Mouse LIF protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0732
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse LIF protein Ser24-Phe203 (Accession# P09056) with a His tag at the C-terminus. |
| GeneID | 16878 |
| Accession | P09056 |
| PredictedSize | 20.9 kDa |
| SDS-PAGE | 30-45 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. It utilises a receptor that consists of the LIF receptor B and gp130 and this receptor complex is also used by ciliary neurotrophic growth factor (CNTF), oncostatin M, cardiotrophin1 (CT1) and cardiotrophin-like cytokine (CLC). Functionally, LIF has been implicated in a many physiological processes including the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
References:
1. D J Hilton.et al. (1991)J Cell Biochem.46(1):21-6. 2. Nicos A.et al. (2005) Cytokine & Growth Factor Reviews.26(5):533-544. 3. C J Auernhammer.et al. (2000) Endocr Rev.21(3):313-45.