Recombinant Mouse Lepr protein (rFc Tag) (HPLC verified)
Species
Mouse
Purity
>90 %, SDS-PAGE
>90 %, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg2930
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Lepr protein Leu22-Gly839 (Accession# P48356-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 16847 |
| Accession | P48356-1 |
| PredictedSize | 118.2 kDa |
| SDS-PAGE | 120-180 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction. Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3. LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity.
References:
1.Cottrell EC. et al. (2012). Handb Exp Pharmacol. (209):3-21. 2.Tartaglia LA. (1997). J Biol Chem. 272(10):6093-6096. 3.Baskin DG. et al. (1999). J Histochem Cytochem. 47(3):353-362.