Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse serum tissue |
| Positive IP detected in | mouse serum |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
| ChIP | See 2 publications below |
| RIP | See 6 publications below |
Product Information
10283-1-AP targets Rabbit anti-Mouse IgG in WB, IHC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Rabbit anti-Mouse IgG |
| Calculated molecular weight | 155 kDa |
| Observed molecular weight | 25 kDa, 50-55 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2877728 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detectes all the heavy and light chains total mouse IgG.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for Rabbit anti-Mouse IgG antibody 10283-1-AP | Download protocol |
| WB protocol for Rabbit anti-Mouse IgG antibody 10283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. | ||
Nucleic Acids Res An H3K4me3 reader, BAP18 as an adaptor of COMPASS-like core subunits co-activates ERα action and associates with the sensitivity of antiestrogen in breast cancer. | ||
J Clin Invest Excessive collagen type VII mediates pleural fibrosis via increasing extracellular matrix stiffness. | ||
Adv Sci (Weinh) The Circadian Transcription Factor CLOCK Modulates Oxidative Stress Resistance via the ACHL-Relish Axis in Drosophila. | ||
Cell Death Differ KMT2D loss drives adeno-to-squamous transition and sensitizes TKI-resistant lung cancer to AURKA inhibition | ||
Cell Death Dis Decreased ZNF750 promotes angiogenesis in a paracrine manner via activating DANCR/miR-4707-3p/FOXC2 axis in esophageal squamous cell carcinoma. |