Speedy™ Mouse IgE One-Step ELISA Kit
Sensitivity
0.03 ng/mL
Range
0.313-20 ng/mL
Reactivity
Mouse
Cat no : SE60120
Synonyms
Ig epsilon chain C region, IGHE
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse IgE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 380792 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 98%-103% |
| Tissue lysate | 96% | 92%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.73 | 0.14 | 1.60 |
| 2 | 8 | 2.51 | 0.04 | 1.59 |
| 3 | 8 | 1.37 | 0.04 | 2.92 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.63 | 0.17 | 1.97 |
| 2 | 16 | 2.53 | 0.05 | 1.98 |
| 3 | 16 | 1.36 | 0.04 | 2.94 |
Background Information
Immunoglobulin E (IgE) is a specialized type of antibody produced by the immune system that plays a pivotal role in defending against parasitic infections (such as helminths) and mediating allergic reactions. Although it is the least abundant antibody isotype in human serum, IgE triggers powerful immune responses by binding to high-affinity receptors on mast cells and basophils. Upon subsequent exposure to a specific allergen, this binding prompts the rapid release of inflammatory chemical mediators like histamine, leading to the classic symptoms of allergic asthma, hay fever, and anaphylaxis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ig epsilon chain C region, IGHE |