Mouse IL-4 ELISA Kit
Cat no : KE10010
Synonyms
BSF-1, Il 4, Il4, IL-4, interleukin 4, Interleukin-4
Validation Data Gallery
Product Information
KE10010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-4. The assay recognizes mouse IL-4. An antibody specific for mouse IL-4 has been pre-coated onto the microwells. The mouse IL-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-4 is added to detect the captured mouse IL-4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16189 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 89% | 72%-98% |
| Cell culture supernatants | 103% | 87%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 100.2 | 2.5 | 2.5 |
| 2 | 20 | 207.7 | 5.9 | 2.9 |
| 3 | 20 | 445.8 | 8.5 | 1.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 104.0 | 3.5 | 3.4 |
| 2 | 24 | 207.0 | 9.1 | 4.4 |
| 3 | 24 | 392.4 | 20.5 | 5.2 |
Background Information
Interleukin-4 (il-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. Il4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. Il4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BSF-1, Il 4, Il4, IL-4, interleukin 4, Interleukin-4 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Bioact Mater Sulfated chitosan enhances BMP-2-mediated spinal fusion via skeletal stem cell rejuvenation in aging. | ||
Nat Commun β(2)-adrenergic signaling controls neonatal respiratory syncytial virus infection by promoting viral clearance and airway protection. | ||
Redox Biol BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis. | ||
J Nanobiotechnology NOS-like activity of CeO(2) nanozymes contributes to diminishing the vascular plaques. | ||
Theranostics Magnetic sculpture-like tumor cell vaccines enable targeted in situ immune activation and potent antitumor effects | ||
Acta Pharm Sin B Targeted intestinal barrier repair via probiotic-derived engineered outer membrane vesicles: A 3A1M strategy with antioxidant, anti-inflammatory, anti-ferroptotic, and microbiome modulation effects. |