Recombinant Mouse IL3RA/CD123 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6614
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse IL-3RA protein Ser17-Lys331 (Accession# P26952) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 16188 |
| Accession | P26952 |
| PredictedSize | 60.5 kDa |
| SDS-PAGE | 70-95 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
IL-3RA, also named as CD123 and SUT-1, is the alpha subunit of the interleukin 3 (IL-3) receptor, which is part of the type I cytokine receptor superfamily. It can regulate the proliferation, survival, and differentiation of hematopoietic cells. In mouse, there are two classes of high-affinity IL-3 receptors. One contains an IL-3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL-5 and GM-CSF receptors.
References:
1.Broughton, Sophie E et al. Cell reports vol. 8,2 (2014): 410-9. 2.Aldoss, Ibrahim et al. Hum Vaccin Immunother. 2020;16(10):2341-2348.