Recombinant Mouse IL-9 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3827
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse IL-9 protein Gln19-Pro144 (Accession# P15247) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 16198 |
| Accession | P15247 |
| PredictedSize | 40.4 kDa |
| SDS-PAGE | 48-62 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH 8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.
References:
1. Ann S Heiman. et al. (2005)J Interferon Cytokine Res. 25(2):82-91. 2. Nicola L Diny. et al. (2016) Eur J Immunol. 46(12): 2749-2760. 3. Petronela Ancuta. et al. (2006) J Immunol. 176(10): 5760-71. 4. Witold Owczarek. et al. (2010) Cytokine. 50(2):181-5.
