Mouse IL-6R alpha ELISA Kit
Cat no : KE10111
Synonyms
CD126, IL 6R, Il6r, Il6ra, interleukin 6 receptor, alpha
Validation Data Gallery
Product Information
KE10111 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-6R alpha ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-6R alpha. The assay recognizes mouse IL-6R alpha. An antibody specific for mouse IL-6R alpha has been pre-coated onto the microwells. The mouse IL-6R alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-6R alpha is added to detect the captured mouse IL-6R alpha protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-6R alpha ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 8.3 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16194 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 91% | 73%-118% |
| Cell culture supernatant | 95% | 77%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,951.7 | 168.0 | 8.6 |
| 2 | 20 | 472.8 | 21.4 | 4.5 |
| 3 | 20 | 107.4 | 8.3 | 7.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,948.0 | 174.7 | 9.0 |
| 2 | 24 | 477.8 | 36.9 | 7.7 |
| 3 | 24 | 126.9 | 11.4 | 9.0 |
Background Information
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by various cells during infection, trauma, and immunological challenges. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6 receptor α (IL-6Rα) subunit (CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6Rα associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). IL-6Rα is produced by proteolytic shedding and alternative splicing to produce soluble IL-6Rα.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD126, IL 6R, Il6r, Il6ra, interleukin 6 receptor, alpha |
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Sci (Weinh) Anti-Inflammatory Mechanisms of Selenium Nanosheets in Ulcerative Colitis: Protein Corona, GP130 Interaction, and Transcriptomic Profile |