Mouse IL-1R1 ELISA Kit
Sensitivity
29.1 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : KE10169
Synonyms
CD121 antigen-like family member A, CD121a, EC:3.2.2.6, Il1r1, IL-1R-1
Validation Data Gallery
Product Information
KE10169 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1R1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1R1. The assay recognizes mouse IL-1R1. An antibody specific for mouse IL-1R1 has been pre-coated onto the microwells. The mouse IL-1R1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-1R1 is added to detect the captured mouse IL-1R1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1R1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 29.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16177 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 93%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,406.9 | 43.3 | 1.8 |
| 2 | 8 | 588.4 | 27.4 | 4.7 |
| 3 | 8 | 326.5 | 14.9 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,410.7 | 41.5 | 1.7 |
| 2 | 16 | 579.4 | 26.5 | 4.6 |
| 3 | 16 | 323.2 | 12.9 | 4.0 |
Background Information
The IL-1R family plays roles in the regulation of innate inflammation, host defense, and acquired immunity. Interleukin-1 receptor type 1 (IL-1R1) is expressed by all cells responsive to IL-1, the predominant type of IL-1R on T cells, fibroblasts, epithelial and endothelial cells. IL-1R1 activation promotes Ly6G expressing neutrophil accumulation in the injured kidney and generation of tumor necrosis factor (TNF) by intrarenal myeloid cells. The soluble form of IL-1R1 acts as a decoy inhibitor by capturing IL-1 cytokines in solution.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD121 antigen-like family member A, CD121a, EC:3.2.2.6, Il1r1, IL-1R-1 |