Purchase

Recombinant Mouse Haptoglobin protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1197


Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity
Not tested
Expression HEK293-derived Mouse Haptoglobin protein Val19-Asn347 (Accession# Q61646) with a His tag at the C-terminus.
GeneID 15439
Accession Q61646
PredictedSize 37.9 kDa
SDS-PAGE 45-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Haptoglobin(HP), also named as zonulin, is a plasma glycoprotein and belongs to the peptidase S1 family. As a kind of acute-phase response protein, serum Hp is mainly synthesized by hepatocytes and is widely distributed in the circulation in humans. The main physiological function of serum Hp is binding free hemoglobin released from red blood cell lysis and protecting tissues and vessels from oxidative damage. The level of Hp is a useful biomarker for some diseases diagnosis, such as Hepatocellular carcinoma, Pancreatic cancer, Type 2 diabetes mellitus, Diabetes kidney disease, and so on.

References:

1. Andersen C B, et al. (2017). Antioxidants & Redox Signaling. 26(14):814. 2. Alayash A I, et al. (2013).Trends in Biotechnolog. 31(1):2-3. 3. Wang S, et al. (2019). Cardiovascular Diabetolog. 18(1). 4. Moriwaki K, et al. (2006). Japan Journal of Molecular Tumor Marker Research. 21(11):2803-2808. 5. Dalal K, et al. (2019). Clinica Chimica Acta. 495:309-317. 6. Shinton N K, et al. (1965). Journal of Clinical Pathology. 18(1):114-118. 7. Bhensdadia N M, et al. (2013). Kidney International. 83(6):1136-1143. 8. Gurung R L, et al. (2018). Scientific Reports. 8(1):5109.


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