Recombinant Mouse Fc-epsilon RI-alpha (FcERI) protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1740
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Fc-epsilon RI-alpha (FcERI) protein Ala24-Gln204 (Accession# P20489) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 14125 |
| Accession | P20489 |
| PredictedSize | 47.3 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, also known as high affinity immunoglobulin epsilon receptor subunit alpha, FCER1A and FCE1A, is a single-pass type I membrane protein which contains 2 immunoglobulin-like domains. FCER1A is a subunit of the IgE receptor, which is composed of one glycosylated alpha (FCER1A), one beta (FCER1B), and two gamma (FCER1G) subunits. The high affinity IgE receptor plays a central role in allergic disease, coupling allergen and mast cells to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
References:
1. S S Saini, et al. (2001) J Allergy Clin Immunol. May;107(5):832-41. 2. Natalija Novak, et al. (2003) J Clin Invest. Apr;111(7):1047-56. 3. M C Seminario, et al. (1999) J Immunol. Jun 1;162(11):6893-900.