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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse Ereg protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2863

Validation Data Gallery

  • Purity of Recombinant Mouse Ereg was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Ereg was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse Ereg was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse Ereg was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90%, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Ereg protein Val56-Leu101 (Accession# Q61521) with a rabbit IgG Fc tag at the C-terminus.
GeneID 13874
Accession Q61521
PredictedSize 31.4 kDa
SDS-PAGE 33-36 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ereg, or epiregulin, a member of the EGF family, binds to the epidermal growth factor receptor (EGFR) and ErbB4, and it stimulates EGFR-related downstream pathways. Previous studies have shown that exposure to various chemical carcinogens induces the EREG expression that is relevant to lung tumorigenesis. The exposure of human bronchial epithelial cells to cigarette smoke extract upregulates EREG, which in turn promotes cell proliferation and protects against cigarette smoke-induced cytotoxicity.

References:

1. Sunaga N. et al. (2024). Cancers (Basel). 16(4):710. 
2. Xu X. et al. (2017). Carcinogenesis. 38(6):604-614.
3. Xiong Y. et al. (2021). JTO Clin Res Rep. 2(7):100181.