Recombinant Mouse Epha2 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg8778
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Epha2 protein Lys26-Val538 (Accession# Q03145) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 13836 |
| Accession | Q03145 |
| PredictedSize | 82.4 kDa |
| SDS-PAGE | kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
EPHA2 (Eph receptor A2) is a type I single-transmembrane protein belonging to the Ephrin receptor subfamily of the receptor tyrosine kinase (RTK) family. This protein consists of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular kinase domain, and is widely expressed on the surface of epithelial cells, endothelial cells, and various tumor cells. Under physiological conditions, EPHA2 participates in cell migration, adhesion, angiogenesis, and tissue boundary formation by binding to its ligands, such as Ephrin-A1. Under pathological conditions, abnormalities in the Epha2 signaling pathway are closely related to tumor progression, metastasis, and drug resistance.
References:
1. Irie, Naoko et al. The Journal of biological chemistry vol. 284,21 (2009): 14637-44. 2. Guo, Hong et al. Cancer research vol. 66,14 (2006): 7050-8. 3. Brantley-Sieders, Dana M et al. Journal of cell science vol. 117,Pt 10 (2004): 2037-49.