Product Information
Product Name: SpeedyPLEX Mouse Cytokine (4-plex) CBA Assay Panel
Sample type: Serum, Plasma and Cell culture supernatant
Test number: 96 tests
| Target name | Standard Curve Range (pg/mL) | Sensitivity (pg/mL) |
| IL-6 | 15.93-5000 | 16.78 |
| IL-10 | 39.06-10000 | 51.09 |
| IL-1 beta | 7.81-2000 | 6.02 |
| TNF-alpha | 7.81-2000 | 3.13 |
Product Components
Name | Size | Quantity | Storage |
Microplate - 96-Well Plate (Flat bottom)1 | 8 holes × 12 strips | 1 plate | Unopened Kit: Store all reagents at 2-8°C for 12 months. Please use a new standard for each assay. |
Microplate - 96-Well Plate (V-bottom)2 | 8 holes × 12 strips | 1 plate | |
Setup Beads Cocktail* | 500 uL/vial | 1 vial | |
PE Control Beads* | 500 uL/vial | 1 vial | |
Protein Standard Cocktail; lyophilized | - | 2 bottles | |
Capture Beads Cocktail (50x)* | 120 uL/vial | 1 vial | |
Biotinylated Detection Antibody Cocktail (50x)* | 120 uL/vial | 1 vial | |
Sample Diluent PT 4B1-af | 30 mL/bottle | 1 bottle | |
Detection Diluent | 30 mL/bottle | 1 bottle | |
Wash Buffer Concentrate (20x) | 30 mL/bottle | 1 bottle | |
Streptavidin-PE (50x)* | 120 uL/vial | 1 vial | |
Plate Cover Seals | - | 4 pieces |
1: Microplate - 96-Well Plate (Flat bottom) match with Hand-held microplate magnet or platewasher with a magnetic platform in the wash step.
2: Microplate - 96-Well Plate (V-bottom) matches with centrifugal machine in the wash step.
* Centrifugation immediately before use.
Sample values
Mouse serum - twelve samples were evaluated for detectable levels of Mouse IL-6, Mouse IL-10, Mouse IL-1 beta and Mouse TNF-alpha in this assay, all samples measured less than the lowest sta-ndard.
one mice were each injected with 15 ug of LPS and were bled 2 hours after injection. Mouse serum samples were evaluated for the presence of IL-6, IL-10, IL-1 beta, TNF-alpha in this assay.
IL-6 (pg/mL) | IL-10 (pg/mL) | IL-1 beta (pg/mL) | TNF-alpha (pg/mL) | |
| LPS injected mouse serum | 66531.7 | 29289.8 | 76.0 | 6652.0 |
Cell culture supernatant:
EL-4 mouse lymphoblast cells (9 x 105cells/mL) were cultured for 2 days in DMEM supplemented with 10% fetal bovine serum and stimulated with 10 ug/mL PHA and 10 ng/mL PMA. An aliquot o-f the culture supernate was removed, assayed for IL-6, IL-10, IL-1 beta, TNF-alpha.
Condition | IL-6 (pg/mL) | IL-10 (pg/mL) | IL-1 beta (pg/mL) | TNF-alpha (pg/mL) |
| Unstimulated (2days) | ND | ND | ND | ND |
| Stimulated (2days) | 59.1 | ND | ND | 10014.4 |
SP2/0 Mouse Myeloma cells (1 x 106 cells/mL) were cultured for 3 days in RPMI with 10% fetal bovine serum, 50 uM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 ug/mL st-reptomycin sulfate. The cell culture supernatant was assayed for IL-6, IL-10, IL-1 beta, TNF-alpha.
IL-6 (pg/mL) | IL-10 (pg/mL) | IL-1 beta (pg/mL) | TNF-alpha (pg/mL) | |
| SP2/0 | ND | 7832 | ND | ND |
J774.A1 mouse mononuclear macrophages cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum. The cells were cultured unstimulated or stimulated with 2.5 ug/mL LPS and 100 ng/mL GM-CSF for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of IL-6, IL-10, IL-1 beta, TNF-alpha.
| Condition | IL-6 (pg/mL) | IL-10 (pg/mL) | IL-1 beta (pg/mL) | TNF-alpha (pg/mL) |
| Unstimulated (3 days) | ND | ND | ND | 154.1 |
| Stimulated (3 days) | 13332.7 | 137.2 | 226.0 | 2306.3 |
ND*=Non-detectable
Cited in Article as
kmf10001, SpeedyPLEX Mouse Cytokine (4-plex) CBA Assay Panel, Proteintech, IL, USA