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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse CXCL5 protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1636

Validation Data Gallery

  • Purity of Recombinant Mouse CXCL5 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CXCL5 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CXCL5 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CXCL5 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CXCL5 protein Val45-Ala118 (Accession# P50228) with a rabbit IgG Fc tag at the C-terminus.
GeneID 20311
Accession P50228
PredictedSize 34.3 kDa
SDS-PAGE 33-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CXCL5 is a member of the ELR+ CXC chemokine family, whose members contain a highly conserved three amino acid motif (ELR+) that promotes angiogenesis and is highly associated with aberrant angiogenesis. CXCL5 is an important chemokine secreted by immune cells, such as monocytes and T lymphocytes. Recent studies indicated that CXCL5 is aberrantly expressed in >14 different types of cancer, including hepatocellular carcinoma, prostate cancer, pancreatic cancer, and gastric cancer. Antibody neutralization of CXCL5 in experimental models of human non-small-cell lung cancer decreased tumor angiogenesis and metastasis.

References:

1. Robert M Strieter.et al. (2004) Semin Cancer Biol. 14(3):195-200. 
2. R M Strieter.et al. (1995) J Biol Chem. 270(45):27348-57.
3. Morgan O'Hayre.et al. (2008) BiochemJ. 409(3):635-49. 
4. Gildas Lepennetier.et al. (2019) J Neuroinflammation. 16(1):219. 
5. Wen Zhang. Et al. (2020) Cancer Commun (Lond). 40(2-3):69-80.