Recombinant Mouse CXCL16 protein (rFc Tag) (HPLC verified)
Species
Mouse
Purity
>90 %, SDS-PAGE
>90 %, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg2846
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CXCL16 protein Asn27-Pro114 (Accession# Q8BSU2) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 66102 |
| Accession | Q8BSU2 |
| PredictedSize | 36.0 kDa |
| SDS-PAGE | 35-42 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 30mM Tris,500mM arginine, pH 8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CXC motif chemokine ligand 16 (CXCL16) is classified as an α sub-family chemokine because it has a CXC motif at the N-terminus. Nevertheless, this chemokine lacks the ELR motif, which is common in other pro-angiogenic CXC chemokines. CXCL16 is expressed in lymphoid organs such as the thymus, spleen, lymph nodes, and Peyer’s patches, but not in bone marrow. It is also expressed in the liver, lung, small intestine, and kidney.
References:
1. Korbecki J, et al. (2021) Int J Mol Sci. 28;22(7):3490. 2. Matloubian M, et al. (2000) Nat. Immunol. 298–304. 3. Wilbanks A, et al. (2001) J. Immunol. 66:5145–5154.