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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse Cfd protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3077

Validation Data Gallery

  • Purity of Recombinant Mouse Cfd was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Cfd was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Cfd protein Gln21-Ser259 (Accession# P03953-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 11537
Accession P03953-1
PredictedSize 52.1 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cfd is a crucial component of the complement system, playing a significant role in host defense and immune regulation. The protein encoded by the Cfd gene is a member of the chymotrypsin family of serine proteases. It plays an essential role in host defense as the rate-limiting enzyme in the alternative pathway of complement activation. Complement factor D activates a convertase (C3bBb) responsible for cleavage of the complement protein C3, which leads to the activation of terminal complement component C5-9 to form the membrane attack complex on microbial or cellular surfaces. Cfd knockout mice display impaired alternative pathway activation of the complement system and increased susceptibility to pneumococcal infection. These mice have a defect in the activation of the alternative pathway, leading to a reduced ability to clear pathogens. Mouse models have been used to study the function of Cfd in various disease states, and its human ortholog CFD is a promising candidate for the development of therapeutic strategies for metabolic and inflammatory diseases.

References:

1. Wang L, Gao P, et al. (2023) J Cachexia Sarcopenia Muscle. 14(5):2152-2167. 
2. Cho M, Hwang JS, et al. (2024) Int J Mol Sci. 25(18):9877. 
3. Ito S, Hashimoto H, et al. (2022) Nat Commun. 13(1):5409. 
4. Ma L, Gilani A, et al. (2024) JCI Insight. 9(11):e178925.