Mouse CXCL1 ELISA Kit

Sensitivity

6.5 pg/mL

Range

31.25-1000 pg/mL

Reactivity

Mouse

Cat no : KE10019

Synonyms

C-X-C motif chemokine 1, Cxcl1, Fsp, gro, Gro1, GRO-alpha, KC, Mgsa, N51, NAP-3, Scyb1



Product Information

KE10019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CXCL1. The assay recognizes mouse CXCL1. An antibody specific for mouse CXCL1 has been pre-coated onto the microwells. The mouse CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CXCL1 is added to detect the captured mouse CXCL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse CXCL1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 6.5 pg/mL
Range 31.25-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 14825

Recovery

Sample TypeAverageRange
Mouse serum90%71%-118%
Cell culture supernatants99%72%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12030.11.03.3
220120.73.52.9
320481.318.03.7

InterAssay

Samplenmean ( pg/mL)SDCV%
12431.81.75.2
224130.14.93.8
324505.014.62.9

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms C-X-C motif chemokine 1, Cxcl1, Fsp, gro, Gro1, GRO-alpha, KC, Mgsa, N51, NAP-3, Scyb1

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

Cell Res

Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects.

Authors - Qiansen Zhang
mousecell culture supernatants

Cancer Lett

Myeloid-derived suppressor cells deficient in cholesterol biosynthesis promote tumor immune evasion

Authors - Yu Chen
mouseserum

J Immunol

HMGB1-Mediated Neutrophil Extracellular Trap Formation Exacerbates Intestinal Ischemia/Reperfusion-Induced Acute Lung Injury.

Authors - YaQing Zhan
mousecell culture supernatants

Am J Physiol Cell Physiol

LRG1 facilitates corneal fibrotic response by inducing neutrophil chemotaxis via Stat3 signaling in alkali-burned mouse corneas.

Authors - Bingjie Yu
mousecell culture supernatants

Inflammation

Silencing TLR4/MyD88/NF-κB Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE.

Authors - Liucheng Wu
mousecell culture supernatants

Exp Ther Med

Ropinirole inhibits inflammatory cytokine production in gingival epithelial cells and suppresses alveolar bone loss in an experimental rat model of periodontitis

Authors - Yuta Isozaki