Speedy™ Mouse CX3CL1 One-Step ELISA Kit
Sensitivity
0.07 ng/mL
Range
0.313-10 ng/mL
Reactivity
Mouse
Cat no : SE60022
Synonyms
ABCD 3, CX3C, Cxc3, Scyd1
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse CX3CL1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate, Tissue homogenate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.313-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20312 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 97% | 88%-107% |
| Tissue lysate | 108% | 102%-120% |
| Tissue homogenate | 111% | 104%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.48 | 0.17 | 3.79 |
| 2 | 8 | 1.13 | 0.06 | 5.31 |
| 3 | 8 | 0.62 | 0.02 | 3.23 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.71 | 0.28 | 5.94 |
| 2 | 16 | 1.20 | 0.10 | 8.33 |
| 3 | 16 | 0.64 | 0.04 | 6.25 |
Background Information
Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. CX3CL1, also known as fractalkine (FKN), is a unique, membrane-bound chemokine, with a transmembrane domain and the chemokine domain on top of a long mucinlike stalk. This protein can exist in two forms, a membrane-bound form or a shed soluble form, after extracellular proteolysis at a membrane-proximal dibasic cleavage site. The soluble CX3CL1 has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes. Presence of CX3CL1 and its receptor CX3CR1 in lymphocytes and their enhanced cellular expression during inflammatory conditions suggests that the CX3CL1-CX3CR1 duo participates in immune-related inflammatory diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ABCD 3, CX3C, Cxc3, Scyd1 |