Speedy™ Mouse CD80/B7-1 One-Step ELISA Kit
Sensitivity
2.0 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Cat no : SE60057
Synonyms
Cd80, B7 1, B7.1, Cd28l, CD80 antigen
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse CD80/B7-1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12519 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 113% | 109%-117% |
| Cell lysate | 101% | 95%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 551.4 | 7.5 | 1.4 |
| 2 | 8 | 130.1 | 3.3 | 2.5 |
| 3 | 8 | 65.4 | 2.3 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 557.0 | 10.6 | 1.9 |
| 2 | 16 | 131.2 | 4.7 | 3.6 |
| 3 | 16 | 68.9 | 4.4 | 6.4 |
Background Information
CD80, also known as B7-1, is a type I membrane protein and a member of the immunoglobulin superfamily. It plays a crucial role in T cell activation and regulation by providing a co-stimulatory signal necessary for T cell proliferation and cytokine production. CD80 interacts with CD28 on T cells to promote T cell activation and with CTLA-4 to inhibit T cell activation. This dual role highlights its importance in modulating immune responses. Additionally, CD80 acts as a receptor for adenovirus subgroup B, indicating its involvement in viral infections.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd80, B7 1, B7.1, Cd28l, CD80 antigen |