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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse CD32B/Fcgr2b protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg5259

Validation Data Gallery

  • Purity of Recombinant Mouse CD32B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD32B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CD32B protein Thr30-Pro210 (Accession# P08101-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 14130
Accession P08101-1
PredictedSize 46.6 kDa
SDS-PAGE 55-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD32B, also known as FcgammaRIIb or FCGR2B, is a protein that belongs to the family of Fc receptors. It is a type I transmembrane protein and a member of the immunoglobulin superfamily of low-affinity immunoglobulin gamma Fc receptors. This protein acts as a low-affinity receptor for the Fc region of immunoglobulin gamma complexes. It plays a crucial role in immune responses, particularly in the phagocytosis of immune complexes. CD32B is involved in several processes, including Fc-gamma receptor signaling pathway involved in phagocytosis, negative regulation of B cell receptor signaling pathway, and regulation of dendritic spine maintenance. It is expressed in various cell types, such as B cells, myeloid cells, and is also found on the surface of B cell leukemia/lymphoma cells. The protein is encoded by the FCGR2B gene and is implicated in various diseases, including autoimmune thrombocytopenic purpura, glomerulonephritis, lymphoid leukemia, malaria, and systemic lupus erythematosus.

References:

1.Stopforth RJ. et al. (2016) J Clin Immunol. 36 Suppl 1:88-94.
2.Tutt AL. et al. (2015) J Immunol. 195(11):5503-16.
3.Lim SH. et al. (2011) Blood. 118(9):2530-40.