Recombinant Mouse CD320 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6327
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CD320 protein Ala29-Gly208 (Accession# Q9Z1P5-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 54219 |
| Accession | Q9Z1P5-1 |
| PredictedSize | 45.1 kDa |
| SDS-PAGE | 58-70 kDa , reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD320 is a type I transmembrane glycoprotein of LDL receptor family functioning as transcobalamin-bound vitamin B12 receptor. It mediates ligand endocytosis to supply cellular cobalamin essential for DNA synthesis and B lymphocyte proliferation in germinal center. Aberrant CD320 mutation causes inherited methylmalonic aciduria, while its overexpression in liver cancer and multiple myeloma fuels malignant proliferation via enhanced B12 intake. Targeted drug conjugates coupled with cobalamin and anti-CD320 antibodies are under preclinical development for tumor-specific chemotherapy, making CD320 a promising delivery and therapeutic target.
References:
1. Quadros, Edward V et al. Blood vol. 113,1 (2009): 186-92. 2. Alam, Amer et al. Nature communications vol. 7 12100. 14 Jul. 2016.