Speedy™ Mouse CD30/TNFRSF8 One-Step ELISA Kit
Sensitivity
14.0 pg/mL
Range
39-2500 pg/mL
Reactivity
Mouse
Cat no : SE60060
Synonyms
CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8, Tumor necrosis factor receptor superfamily member 8
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse CD30/TNFRSF8 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 14.0 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21941 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 91% | 84%-102% |
| Cell culture supernatant | 100% | 97%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,028.2 | 76.4 | 7.4 |
| 2 | 8 | 268.7 | 11.3 | 4.2 |
| 3 | 8 | 140.3 | 7.0 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,027.2 | 73.8 | 7.2 |
| 2 | 16 | 263.6 | 12.4 | 4.7 |
| 3 | 16 | 137.7 | 6.7 | 4.9 |
Background Information
CD30, also known as TNFRSF8, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. It plays a crucial role in immune regulation and signaling. CD30 interacts with its ligand, CD30L (CD153), which is expressed on activated T cells, leading to the activation of downstream signaling pathways such as NF-κB, MAPK, and JAK/STAT. These pathways are involved in cell proliferation, survival, and immune response modulation. In mice, soluble CD30 (sCD30) can be detected in serum and other body fluids. Studies in mice have shown that sCD30 can modulate immune responses by competing with membrane-bound CD30 for CD30L binding, thereby reducing the activation of downstream signaling pathways.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8, Tumor necrosis factor receptor superfamily member 8 |