Recombinant Mouse CD21 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1396
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CD21 protein Ile12-Trp963 (Accession# P19070) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 12902 |
| Accession | P19070 |
| PredictedSize | 130.9 kDa |
| SDS-PAGE | 130-200 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Complement receptor type 2 (CR2, CD21) is a 145-kD glycosylated single polypeptide chain consisting of a long extracellular domain ofshort consensus repeat sequences, a transmembrane region and a short cytoplasmic domain. The receptor is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells. CD21 binds complement fragments C3d, C3dg and iC3b that are covalently bound to target antigens. On B cells, CD21 forms a complex together with CD19 and CD81 (TAPA-1), which functions as a co-receptor to the BCR.
References:
1.Thorarinsdottir, K et al.Scandinavian journal of immunology vol. 82,3 (2015): 254-61. 2.Roozendaal, Ramon, and Michael C Carroll.Immunological reviews vol. 219 (2007): 157-66. 3.Wilbrink, Rick et al.Frontiers in immunology vol. 12 686273. 8 Jun. 2021.