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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CCL19/MIP-3 beta Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 12.5-800 pg/mL

Cat no : MP02165-1

Print datasheet

Synonyms

C-C motif chemokine 19, Ccl19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02165-1, Mouse CCL19/MIP-3 beta Recombinant Matched Antibody Pair - PBS only. 85861-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3124. 85861-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

    Sandwich ELISA standard curve of MP02165-1

    Sandwich ELISA standard curve of MP02165-1, Mouse CCL19/MIP-3 beta Recombinant Matched Antibody Pair - PBS only. 85861-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3124. 85861-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

  • Serum of four mice was measured. The CCL19/MIP-3 beta concentration of detected samples was determined to be 424.5 pg/mL with a range of 338.8-499.6 pg/mL.

    Sample test of MP02165-1

    Serum of four mice was measured. The CCL19/MIP-3 beta concentration of detected samples was determined to be 424.5 pg/mL with a range of 338.8-499.6 pg/mL.

  • Mouse spleen tissue was removed, rinsed in 1x PBS, and kept on ice. The tissue was then homogenized using a tissue homogenizer and seeded into media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for 3 days. Aliquots of the cell culture supernatants were removed and assayed for levels of mouse CCL19/MIP-3 beta, and measured 25.7 pg/mL.

    Sample test of MP02165-1

    Mouse spleen tissue was removed, rinsed in 1x PBS, and kept on ice. The tissue was then homogenized using a tissue homogenizer and seeded into media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for 3 days. Aliquots of the cell culture supernatants were removed and assayed for levels of mouse CCL19/MIP-3 beta, and measured 25.7 pg/mL.

  • Cytometric bead array standard curve of MP02165-1, MOUSE CCL19/MIP-3 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85861-3-PBS. Detection antibody: 85861-1-PBS. Standard: Eg3124. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP02165-1

    Cytometric bead array standard curve of MP02165-1, MOUSE CCL19/MIP-3 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85861-3-PBS. Detection antibody: 85861-1-PBS. Standard: Eg3124. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02165-1 targets CCL19/MIP-3 beta in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 12.5-800 pg/mL (Sandwich ELISA);

Capture antibody: Mouse Ccl19 Recombinant antibody, PBS Only (Capture) 85861-3-PBS (242867E7). 100 μg. Concentration 1 mg/ml.

Detection antibody: Mouse Ccl19 Recombinant antibody, PBS Only (Detector) 85861-1-PBS (242867C1). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg3124

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 85861-3-PBS
Tested Reactivity mouse
Immunogen Ccl19 fusion protein Eg3124
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 19
Synonyms CCL19/MIP-3 beta, C-C motif chemokine 19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine
GenBank accession numberNM_011888.2
Gene symbol Ccl19
UniprotO70460
Gene ID (NCBI) 24047
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 85861-1-PBS
Tested Reactivity mouse
Immunogen Ccl19 fusion protein Eg3124
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 19
Synonyms CCL19/MIP-3 beta, C-C motif chemokine 19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine
GenBank accession numberNM_011888.2
Gene symbol Ccl19
UniprotO70460
Gene ID (NCBI) 24047
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.