KE10051 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL12/MCP-5 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL12/MCP-5. The assay recognizes mouse CCL12/MCP-5. An antibody specific for CCL12/MCP-5 has been pre-coated onto the microwells. The CCL12/MCP-5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL12/MCP-5 is added to detect the captured mouse CCL12/MCP-5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.