Recombinant Mouse C-Reactive Protein/CRP protein (hFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
hFc Tag
Activity
not tested
Cat no : Eg0557
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CRP protein His20-Ser225 (Accession# NP_031794.3) with a human IgG1 Fc tag at the C-terminus. |
| GeneID | 12944 |
| Accession | NP_031794.3 |
| PredictedSize | 49.1 kDa |
| SDS-PAGE | 48-52 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
C-reactive protein (CRP) is an acute-phase serum protein predominately synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.
References:
1. Pepys MB, et al. (2003) C-reactive protein: a critical update.111(12):1805-12. 2. Black S, et al. (2004) C-reactive Protein.279(47):48487-90.