Speedy™ Mouse Angiopoietin-like 3/ANGPTL3 One-Step ELISA Kit
Sensitivity
4.1 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Cat no : SE60016
Synonyms
Angptl3, angiopoietin like 3
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Angiopoietin-like 3/ANGPTL3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 4.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 30924 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 89% | 87%-92% |
| Cell culture supernatant | 86% | 77%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 429.5 | 10.8 | 2.5 |
| 2 | 8 | 115.0 | 8.5 | 7.4 |
| 3 | 8 | 61.8 | 3.5 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 443.0 | 31.0 | 7.0 |
| 2 | 16 | 114.0 | 6.3 | 5.5 |
| 3 | 16 | 60.4 | 3.1 | 5.1 |
Background Information
ANGPTL3 is a secreted glycoprotein primarily synthesized by hepatocytes and belongs to the angiopoietin-like protein family. ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis, and hematopoietic function, and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. ANGPTL3 inhibits low-density lipoprotein (LDL) and endothelial lipase (EL), two enzymes that hydrolyze triglycerides and phospholipids in plasma lipoproteins. ANGPTL3-deficient mice showed low plasma HDL cholesterol and HDL phospholipid (PL). ANGPTL3 can also regulate triglyceride metabolism by inhibiting lipoprotein lipase (LPL). ANGPTL3-deficient mice exhibit hypotriglyceridemia and elevated LPL activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angptl3, angiopoietin like 3 |