Mouse Albumin ELISA Kit
Sensitivity
0.2 pg/mL
Range
23.4-1500 pg/mL
Reactivity
Mouse
Cat no : KE10117
Synonyms
Alb,Alb 1,Alb1,albumin
Validation Data Gallery
Product Information
KE10117 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Albumin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Albumin. The assay recognizes mouse Albumin. An antibody specific for mouse Albumin has been pre-coated onto the microwells. The mouse Albumin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Albumin is added to detect the captured mouse Albumin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Albumin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11657 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 107% | 80%-126% |
| Urine | 96% | 86%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 748.6 | 43.1 | 5.8 |
| 2 | 20 | 172.6 | 3.4 | 1.9 |
| 3 | 20 | 39.1 | 1.5 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 737.3 | 64.6 | 8.8 |
| 2 | 24 | 174.4 | 13.3 | 7.6 |
| 3 | 24 | 44.4 | 4.2 | 9.4 |
Background Information
KE00394 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AFP ELISA kit is to be used to detect and quantify protein levels of endogenous human AFP. The assay recognizes human AFP. An antibody specific for human AFP has been pre-coated onto the microwells. The human AFP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AFP is added to detect the captured human AFP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alb,Alb 1,Alb1,albumin |