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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Mouse Angiotensinogen protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2763

Validation Data Gallery

  • Purity of Recombinant Mouse Angiotensinogen was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Angiotensinogen was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse Angiotensinogen was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse Angiotensinogen was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Angiotensinogen protein Asp25-Val477 (Accession# P11859) with a rabbit IgG Fc tag at the C-terminus.
GeneID 11606
Accession P11859
PredictedSize 75.6 kDa
SDS-PAGE 70-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. AGT is an essential component of the RAS. AGT is cleaved by renin enzyme to produce angiotensin 1 (Ang I) when blood pressure is reduced, and AGT-converting enzyme (ACE) splits Ang I to generate angiotensin 2 (Ang II). RAS plays an important role in maintaining fluid and electrolyte homeostasis, and RAS irregulated expression leads to kidney disease, heart failure, hypertension, and diabetes.

References:

1. Matsusaka T, et al. (2012). J Am Soc Nephrol. 23(7):1181-9. 
2. Lu H, et al. (2016). Hypertens Res.39(7):492-500. 
3. Li S, et al. (2021). Mol Med Rep. 23(3):193.
4. Wegman-Ostrosky T, et al. (2015). J Renin Angiotensin Aldosterone Syst.16(2):227-33.
5. Ames MK, et al. (2019). J Vet Intern Med. 33(2):363-382. 
6. Li XC, et al. (2017). 125(Pt A):21-38.