Speedy™ Mouse Adiponectin One-Step ELISA Kit
Sensitivity
1.4 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Cat no : SE60003
Synonyms
Adipoq, Acrp30, adipo, apM1
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Adiponectin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue homogenate |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11450 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 96%-108% |
| Cell culture supernatant | 107% | 96%-123% |
| Tissue homogenate | 91% | 84%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 477.3 | 14.4 | 3.0 |
| 2 | 8 | 125.0 | 4.5 | 3.6 |
| 3 | 8 | 63.0 | 2.1 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 495.4 | 24.9 | 5.0 |
| 2 | 16 | 127.8 | 6.3 | 4.9 |
| 3 | 16 | 64.1 | 3.1 | 4.8 |
Background Information
Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adipoq, Acrp30, adipo, apM1 |