Speedy™ Mouse APOE One-Step ELISA Kit
Sensitivity
0.03 ng/mL
Range
0.156-10 ng/mL
Reactivity
Mouse
Cat no : SE60015
Synonyms
AI255918, apolipoprotein E
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse APOE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11816 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 92% | 80%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.15 | 0.13 | 2.52 |
| 2 | 8 | 1.25 | 0.02 | 1.60 |
| 3 | 8 | 0.69 | 0.02 | 2.90 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.16 | 0.13 | 2.52 |
| 2 | 16 | 1.24 | 0.02 | 1.61 |
| 3 | 16 | 0.67 | 0.03 | 4.48 |
Background Information
Apolipoprotein E (APOE) is a core component of plasma lipoproteins and is involved in a variety of biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immune regulation, and neuronal growth and repair.The ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Mouse APOE, like ApoE4, contains equivalents of Arg-112 and Glu-255 but lacks the critical Arg-61 equivalent. APOE has heparin-binding activity and binds to heparan sulfate proteoglycans on the cell surface, a property that facilitates cellular capture and receptor-mediated uptake of APOE-containing lipoproteins.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI255918, apolipoprotein E |